PCR DNA extraction and purification magnetic beads kit
This kit provides a simple, rapid and efficient method for the recovery and purification of DNA directly from PCR products (100 bp to 50 kb) with typical recovery efficiency up to 85%. The resulting product can be directly used for sequencing, restriction digestion, or PCR and other downstream experiments. In addition, the kit can be used to concentrate DNA.
The kit will work with a 96 well round bottom plates if a special magnetic frame is used. The kit
can also be used with a variety of automatic nucleic acid extraction instruments and workstation. |
Catalog # |
Si-Mag-PG1 |
Precautions |
- Wear protective gloves, clothing, eye, and face protection. Wash hands thoroughly after handling.
- Avoid freeze/thaw cycles and centrifugation which could damage the beads.
- Vortex samples for about 10 seconds before adding magnetic beads.
- Vortex beads for about 10 seconds and mix them well with DNA containing material to ensure best performance.
- Elute DNA from the beads completely.
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Kit Components included |
- Si-Mag magnetic beads 5 mL
- Elution Buffer 4 mL
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Materials needed but not provided with the kit |
- 80% Ethanol in water.
- Si-Mag Magnet (sold separately) or other magnetic racks compatible with vials used.
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Storage |
Magnetic beads should be stored at 2-8°C but other kit reagents need to be stored at room temperature. |
Protocol |
- Sample preparation. If PCR production size is smaller than 300 bp, add 50 uL of Si-Mag magnetic beads solution to 50 uL of PCR product. But if PCR production size is greater than 300 bp, add 25 uL of Si-Mag magnetic beads solution to 50 uL of PCR product.
- Transfer all content to an Eppendorf tube, mix well and incubate 3-5 min at RT. Then put Eppendorf tube into the Si-Mag magnet rack for 2 min or until solution becomes completely clear. Remove supernatant by holding the magnet rack upside or by pipetting.
- Wash the beads with 200 uL of 80% ethanol twice.
- Dry the beads at RT for 3-5 min leaving the tube open. Do not over-dry the beads.
- Elute the DNA from beads with 20-30 uL of elution buffer, incubate for at least 5 min at RT. Alternatively, incubation at 55°C for 2 min may improve the recovery for DNA larger than 3 kb.
- Remove beads by using magnet rack for 2-3 min, pipette the supernatant DNA out and transfer to a clean tube.
- Store purified DNA at -20°C for long-term storage.
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